Collection And Transport Of Clinical Specimens Pdf

collection and transport of clinical specimens pdf

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Published: 12.05.2021

Public Health Menu. Demonstration of pathogenic organisms in the patient specimen is the most definitive test in microbiology.

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Testing provided through the Unexplained Critical Illness and Deaths Project for unexplained deaths that appear likely to have infectious etiologies will be primarily pathology-directed. Therefore, it is important when possible to provide optimal pathology specimens.

These clinical specimens may be useful in determining an etiology for cases of unexplained illness or death that are likely to have infectious causes. These specimens should be saved for possible cases. While timing of collection and volume of some specimens may not be optimal, it may still be possible to use them for testing. On this page: Types of specimens Specimen collection and handling Laboratory specimens Shipping. Ideally, tissue for CDC would be collected separately at the time of autopsy and would include tissue from each organ and from several areas of each organ.

Fixed tissue from multiple organs can be combined in one container. If specimens are limited, priority should be given to providing fixed tissue, especially from the primary affected organ for example, brain tissue for a meningoencephalitis case.

If a specifically affected area is noted by gross pathology or preliminary histopathology, it is helpful to submit tissue from this area. Each specimen container should be labeled with a case identification number assigned by MDH call for a case number.

Identifying information such as name, address, and medical record number may be removed. Preliminary reports are acceptable. Annual Summary of Communicable Diseases Reported to the Minnesota Department of Health, MDH collects disease surveillance information on certain communicable diseases to determine disease impact, assess trends in disease occurrence, characterize affected populations, prioritize disease control efforts, and evaluate disease prevention strategies.

Clinical Specimen Collection and Handling Unexplained Critical Illnesses and Deaths Testing provided through the Unexplained Critical Illness and Deaths Project for unexplained deaths that appear likely to have infectious etiologies will be primarily pathology-directed. On this page: Types of specimens Specimen collection and handling Laboratory specimens Shipping Types of specimens Two types of specimens are needed in order to provide a complete evaluation: Fixed tissue Fresh frozen tissue Body fluids are also helpful A nasopharyngeal swab can also be collected if a respiratory infection is suspected Specimen collection and handling Ideally, tissue for CDC would be collected separately at the time of autopsy and would include tissue from each organ and from several areas of each organ.

If fixed tissue is submitted to CDC more than 2 weeks after it is collected, paraffin-embedded tissue is preferred for submission. Over-fixation will form a strong formalin bond in tissue and make antigen retrieval more difficult.

Blocks rather than slides are also preferred since epitopes and nucleic acids can degenerate more quickly once tissue is prepared for slides thus decreasing sensitivity of special assays. Fixed tissue should be stored and shipped at room temperature. Do not freeze fixed tissue. Fresh frozen tissue Frozen tissue is used for culture and molecular techniques including specific primer and consensus polymerase chain reaction PCR.

Specimens should be collected aseptically and as soon as possible after death. A separate sterile instrument should be used for each collection site and each specimen should be placed in a separate sterile container in small amounts of viral transport media or saline.

Body fluids cc of cerebrospinal fluid and 5 cc of heart blood in a marbled red tube top collected postmortem may also be used for testing. Body fluid specimens should be kept refrigerated and shipped with a cold pack or dry ice if specimens are being sent directly to CDC. Please refer to the laboratory specimens chart for information on collection and handling of body fluid specimens obtained prior to death.

Nasopharyngeal swab If a respiratory infection is suspected, a postmortem nasopharyngeal swab can be collected and sent to the Minnesota Department of Health MDH in a sterile container with viral transport media for viral culture. Laboratory specimens Laboratory Specimens: Collection and Handling of Clinical Specimens PDF A chart listing clinical specimens, optimal timing of collection, optimal volume, container type, and temperature for storing and shipping.

A list of specimens being sent should also be included. Fixed tissue should be sent at room temperature while frozen tissue and body fluid should be sent on dry ice.

Please call for more information on shipping. Share This Spotlight Annual Summary of Communicable Diseases Reported to the Minnesota Department of Health, MDH collects disease surveillance information on certain communicable diseases to determine disease impact, assess trends in disease occurrence, characterize affected populations, prioritize disease control efforts, and evaluate disease prevention strategies.

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It has as its aim to respond to the challenges currently posed by everything associated with infectious diseases, from a clinical, microbiological and public health perspective. The Journal follows a rigorous selection process of the manuscripts published through the review by the best experts in each area of knowledge of the specialty. The quality of the material published is the main aim of the Editors, as well as to provide readers with the latest and most relevant information in the world of infectious diseases. The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two receding years. CiteScore measures average citations received per document published. Read more.

Testing provided through the Unexplained Critical Illness and Deaths Project for unexplained deaths that appear likely to have infectious etiologies will be primarily pathology-directed. Therefore, it is important when possible to provide optimal pathology specimens. These clinical specimens may be useful in determining an etiology for cases of unexplained illness or death that are likely to have infectious causes. These specimens should be saved for possible cases. While timing of collection and volume of some specimens may not be optimal, it may still be possible to use them for testing.


Instructions should be given to each staff concerned and displayed at specimen collection points. B. Packaging of specimen for transport a. Clinical specimens.


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This article, the first in a four-part series , describes the general principles of specimen collection and how to collect a midstream specimen of urine. Nursing Times [online]; 7, Obtaining a specimen involves the collection of tissue or fluids for laboratory analysis or near-patient testing, and may be the first step in determining a diagnosis and treatment Dougherty and Lister, The procedure used to collect a specimen must minimise the risk of introducing error and protect the health and safety of both the patient and the staff who handle the sample. This article, the first in a four-part series, explores the general principles of specimen collection and describes how to collect a midstream specimen of urine MSU.

SAMPLE COLLECTION, HANDLING AND TRANSPORT

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Blood specimens should be immediately inoculated into a blood culture bottle and transported to a microbiology laboratory as soon as possible for overnight.

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in an appropriate manner, easy-to-under- stand instructions for proper specimen col- lection and transport conditions in the form of a specimen collection manual.

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